Biomarker responses in earthworm Eisenia andrei exposed to pirimiphos-methyl and deltamethrin using different toxicity tests

► Differences in molecular biomarker responses when using different test systems. ► Pirimiphos-methyl causes significant changes in biomarkers in dose-dependent manner. ► Hormetic effect of AChE and CES activity after exposure to pirimiphos-methyl. ► Deltamethrin causes significant changes in biomarkers with no dose response pattern. ► Expected environmental concentrations cause significant changes in biomarkers.