Assessment of aquatic experimental versus predicted and extrapolated chronic toxicity data of four structural analogues

► Daphnia magna highly sensitive to chronic (chlorinated) aniline exposure. ► QSAR inadequate to predict chronic toxicity. ► Inverse relationship of chronic toxicity with log Kow. ► ACR of 10 underestimates chronic toxicity. ► Multi-species extrapolation > individual-based extrapolations.