کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6312645 | 1619034 | 2016 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Plasma levels of selenium-containing proteins in Inuit adults from Nunavik
ترجمه فارسی عنوان
سطوح پلاسمایی پروتئین حاوی سلنیوم در بزرگسالان انویتی از نونویک
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کلمات کلیدی
PTMSELPGPX3Affinity columnKEDCTQMeHgUPLCCRMSeCysInuit - اینویتKinetic energy discrimination - تبعیض انرژی جنبشیMercury - جیوهSeMet - خودشانIsotope dilution - رقیق کردن ایزوتوپSelenomethionine - سلنمتینیونSelenocysteine - سلنوسیستینSelenoprotein P - سلنوپروتئین PSelenoproteins - سلنوپروتئین هاSelenium - سلنیومinductively coupled plasma mass spectrometry - طیفسنجی جرمی پلاسمای جفتشده القاییICP-MS - طیفسنجی جرمی پلاسمای جفتشده القاییArctic - قطب شمالMethylmercury - متیل کرکریultra performance liquid chromatography - کروماتوگرافی مایع با عملکرد فوق العادهGlutathione peroxidase 3 - گلوتاتیون پراکسیداز 3
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
شیمی زیست محیطی
چکیده انگلیسی
Selenium (Se) is highly abundant in marine foods traditionally consumed by Inuit of Nunavik (Northern Quebec, Canada) and accordingly, their Se intake is among the highest in the world. However, little is known regarding the biological implications of this high Se status in this Arctic indigenous population. We used a method combining affinity chromatography and inductively coupled plasma-mass spectrometry with quantification by post-column isotope dilution to determine total Se levels and concentrations of Se-containing proteins in archived plasma samples of Inuit adults who participated to the 2004 Nunavik Inuit Health Survey (N = 852). Amounts of mercury (Hg) associated with Se-containing proteins were also quantified. Results show that glutathione peroxidase 3 (GPx3), selenoprotein P (SelP) and selenoalbumin (SeAlb) represented respectively 25%, 52% and 23% of total plasma Se concentrations. In addition, small amounts of Hg co-eluted with each Se-containing protein and up to 50% of plasma Hg was associated to SelP. Total plasma Se concentrations (median = 139 μg Lâ 1; interquartile range (IQR) = 22.7 μg Lâ 1) were markedly lower and less variable than whole blood Se concentration (median = 261 μg Lâ 1, IQR = 166 μg Lâ 1). A non linear relation was observed between whole blood Se and plasma Se levels, with plasma Se concentrations leveling off at approximately 200 μg Lâ 1, whereas 16% and 3% of individuals exhibited whole blood concentrations higher than 500 μg Lâ 1 and 1000 μg Lâ 1, respectively. In contrast, a linear relationship was previously reported in communities consuming Brazil nuts which are rich Se, mainly present as selenomethionine. This suggests that a different selenocompound, possibly selenoneine, is present in the Arctic marine food chain and accumulates in the blood cellular fraction of Inuit.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Environment International - Volume 96, November 2016, Pages 8-15
Journal: Environment International - Volume 96, November 2016, Pages 8-15
نویسندگان
Adel Achouba, Pierre Dumas, Nathalie Ouellet, Mélanie Lemire, Pierre Ayotte,