کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6321838 | 1619726 | 2016 | 12 صفحه PDF | دانلود رایگان |
- Metabolites of Gammarus pulex were determined following liquid chromatography coupled high resolution mass spectrometry.
- The toxicity study of 26 pharmaceuticals in Gammarus pulex show values between 0.57 mg Lâ 1 to > 250 mg Lâ 1.
- Variations in the metabolite concentrations were detected in the pharmaceuticals exposed samples respect to control samples.
- Pathway alterations related to protein synthesis, oxidative stress and signaling cascades were observed in exposed samples.
The effects of pharmaceuticals and personal care products (PPCPs) on aquatic organisms represent a significant current concern. Herein, a targeted metabolomics approach using liquid chromatography-high resolution mass spectrometry (LC-HRMS) is presented to characterise concentration changes in 29 selected metabolites following exposures of aquatic invertebrates, Gammarus pulex, to pharmaceuticals. Method performance revealed excellent linearity (R2 > 0.99), precision (0.1-19%) and lower instrumental limits of detection (0.002-0.20 ng) for all metabolites studied. Three pharmaceuticals were selected representing the low, middle and high range of measured acute measured toxicities (of a total of 26 compounds). Gammarids were exposed to both the no-observed-adverse-effect-level (NOAEL) and the lowest-observed-adverse-effect-level (LOAEL) of triclosan (0.1 and 0.3 mg Lâ 1), nimesulide (0.5 and 1.4 mg Lâ 1) and propranolol (100 and 153 mg Lâ 1) over 24 h. Quantitative metabolite profiling was then performed. Significant changes in metabolite concentrations relative to controls are presented and display distinct clustered trends for each pharmaceutical. Approximately 37% (triclosan), 33% (nimesulide) and 46% (propranolol) of metabolites showed statistically significant time-related effects. Observed changes are also discussed with respect to internal concentrations of the three pharmaceuticals measured using a method based on pulverised liquid extraction, solid phase extraction and LC-MS/MS. Potential metabolic pathways that may be affected by such exposures are also discussed. This represents the first study focussing on quantitative, targeted metabolomics of this lower trophic level benthic invertebrate that may elucidate biomarkers for future risk assessment.
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Journal: Science of The Total Environment - Volume 562, 15 August 2016, Pages 777-788