کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
636405 1456129 2010 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Surface modification of microporous PVDF membranes for neuron culture
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تصفیه و جداسازی
پیش نمایش صفحه اول مقاله
Surface modification of microporous PVDF membranes for neuron culture
چکیده انگلیسی
Microporous poly(vinylidene fluoride) (PVDF) membranes with either dense or porous surface were prepared by isothermal immersion-precipitation of a casting solution in coagulation baths of different strengths. Onto the membrane surface, amino acid (l-lysine) or 1,6-hexanediamine (HMDA) was chemically immobilized. The membrane was first grafted with poly(glycidyl methacrylate) (PGMA) by means of plasma-induced free radical polymerization. Then, l-lysine or 1,6-hexanediamine was reacted with epoxy groups in the grafted PGMA to create an ECM environment suited to cell culture. Neuronal cells were cultivated on the formed pristine and surface-modified PVDF membranes. It is found that neurons tend to aggregate into large clusters with neuritic branches on pristine PVDF membranes, whereas for those cultured on the PGMA/PVDF membranes, serious aggregation takes place as well, but without neurites being formed. In contrast, neurons did not aggregate on the l-lysine or HMDA-immobilized membrane. It is also interesting to find that a neuritic network inter-connecting cells is constructed for the former, yet no neurite is observed for the latter membrane. Such phenomenon is thought to be associated with the extra -COOH group in l-lysine with respect to HMDA.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Membrane Science - Volume 350, Issues 1–2, 15 March 2010, Pages 32-41
نویسندگان
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