کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6376082 | 1624838 | 2015 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Agrobacterium tumefaciens mediated genetic transformation of Ocimum gratissimum: A medicinally important crop
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کلمات کلیدی
5-Bromo-4-chloro-3-indolyl-β-D-glucuronideNaAAcetosyringoneBAPIBAAgrobacterium tumefaciens mediated transformationX-GlucSMMnptIIβ-glucuronidase - β-گلوکورونیدازAtMT - اتمindole-3-butyric acid - اسید Indole-3-ButyricBenzylaminopurine - بنزیلامینوپورینGenetic transformation - تحول ژنتیکیEssential oil - عطرمایه یا اسانسNeomycin phosphotransferase - فوموتانسفراز نئومیسینnaphthalene acetic acid - نفتالین استیک اسیدgusA - گواتر
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The present report focuses on the development of an efficient protocol for direct regeneration and Agrobacterium mediated genetic transformation of O. gratissimum. CN; when inoculated on MS + 1.5 mg/L + 0.5 mg/L + 0.5 mg/L KN medium has shown the best response with a regeneration frequency of 98% ± 0.4. A. tumefaciens LBA4404 containing binary vector pBI121, harboring gus-A reporter gene with intron under the transcriptional control of Cauliflower Mosaic Virus (CaMV) 35S promoter and neomycin phosphotransferase (npt-II) as selection marker genes, to achieve maximum transformation frequency (20% ± 0.7). To carry out transformation, parameters like O.D., infection duration, concentration of AS, surfactant, (O.D600-0.6, 15 min, 300 μM, 0.01%) were standardized. The putative transformants were screened on SMM having 50 mg/L kanamycin and subsequently rooted on ½ MS. The confirmation was done through PCR. The established regeneration and transformation protocol could be used as a tool to elucidate the functional genomics of important/novel compound which can be used in the preparation of therapeutic formulations for future.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Industrial Crops and Products - Volume 71, September 2015, Pages 138-146
Journal: Industrial Crops and Products - Volume 71, September 2015, Pages 138-146
نویسندگان
Sana Khan, Naveera Fahim, Pooja Singh, Laiq Ur Rahman,