کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
639018 | 1456185 | 2006 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Hydrolysis of whey protein isolate in a tangential flow filter membrane reactor
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کلمات کلیدی
Residual enzyme activityMultivariate data analysis - تجزیه و تحلیل داده های چند متغیرهPrincipal component analysis - تحلیل مولفههای اصلی یا PCAEnzymatic membrane reactor - راکتور غشایی آنزیمیResponse surface methodology - روش سطح پاسخEnzyme inactivation - غیر فعال شدن آنزیمEnzyme leakage - نشت آنزیم
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تصفیه و جداسازی
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
The fate of Protease N (IUB 3.4.24.28, Bacillus subtilis) enzyme was monitored while being used to hydrolyse an initial concentration of 5% (w/v) whey protein isolate (86.98% Kjeldahl nitrogen Ã 6.38) continuously for 5 h at pH 7.0 and 55 °C in an enzymatic membrane reactor (EMR) fitted with a 10 kDa tangential flow filter (TFF). The retentate temperature (A: 25-55 °C), initial water permeate flux, Ji (B: 1.6-18.4 mL/min) and enzyme concentration (C: 0.5-5.5 g) were varied and optimised using response surface methodology (RSM) central composite rotatable design (CCRD). The residual enzyme activity (Aresidual), enzyme leakage (Aleakage), enzyme loss (Aloss), average permeate flux (Javerage) and nitrogen recovered in permeate (apparent sieving, Sapparent) were determined. Aleakage was independent of enzyme concentration but increased concomitant with increasing A and B, while both Aresidual and Aloss decreased with increasing Ji. Protease N was inhibited by WPI and hydrolysates. At 50 °C Protease N enzyme solubilised the concentration polarisation layer (GPL), stabilised Javerage and led to higher Aleakage. Principal components analysis isolated the EMR hydrodynamics due to the retentate temperature, Sapparent, Aleakage and Javerage as factors providing prominent influence in the EMR (principal components 1 and 3 which caused ca. 60% of the EMR variance) while principal component 2 ('measure' of Protease N enzyme property within the reactor) contributed 27.78%. The fate of the enzyme was accounted for as a balance between Aresidual, Aleakage and Aloss.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Membrane Science - Volume 286, Issues 1â2, 15 December 2006, Pages 322-332
Journal: Journal of Membrane Science - Volume 286, Issues 1â2, 15 December 2006, Pages 322-332
نویسندگان
Seronei Chelulei Cheison, Zhang Wang, Shi-Ying Xu,