کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6395433 1628473 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Wine lees valorization: Biorefinery development including production of a generic fermentation feedstock employed for poly(3-hydroxybutyrate) synthesis
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Wine lees valorization: Biorefinery development including production of a generic fermentation feedstock employed for poly(3-hydroxybutyrate) synthesis
چکیده انگلیسی

This study demonstrates the development of a novel wine lees (WL) based integrated biorefinery for the production of several added-value products. WL were initially fractionated for the production of antioxidants, tartrate and ethanol and the remaining stream was converted into a fermentation nutrient supplement for poly(3-hydroxybutyrate) (PHB) production using the strain Cupriavidus necator DSM 7237. Hydrolysis of pretreated WL was carried out using crude enzyme consortia produced via solid state fermentation of Aspergillus oryzae. Optimization of hydrolysis was based on the enhancement of total Kjeldahl nitrogen to free amino nitrogen (FAN) conversion yield by evaluating the effect of the initial pH value, temperature, initial proteolytic activity and initial WL concentration. WL hydrolysates and crude glycerol were used as nutrient and carbon sources, respectively, in batch and fed-batch fermentations for the production of PHB. Bacterial growth and PHB production were influenced significantly by the FAN content of the WL derived hydrolysates and by the addition of trace elements. Using an initial FAN concentration of 700 mg L− 1 and supplementation with trace elements led to the production of 30.1 g L− 1 of PHB concentration with an intracellular content of 71.3% (w w-1) and a productivity of 0.56 g L− 1 h− 1 during fed-batch fermentation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Research International - Volume 73, July 2015, Pages 81-87
نویسندگان
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