Barcode High Resolution Melting analysis for forensic uses in nuts: A case study on allergenic hazelnuts (Corylus avellana)

► Novel specific and highly sensitive HRM based method using chloroplast regions. ► Bar-HRM, with universal and specific chloroplast primers, for barcoding dried nuts. ► We applied this method applied to processed nut products such as biscuits. ► We detected a ratio of 0.01% of hazelnut in biscuits.