کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6451185 1361334 2017 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research review paperImproving the secretory capacity of Chinese hamster ovary cells by ectopic expression of effector genes: Lessons learned and future directions
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Research review paperImproving the secretory capacity of Chinese hamster ovary cells by ectopic expression of effector genes: Lessons learned and future directions
چکیده انگلیسی

Chinese hamster ovary (CHO) cells are the preferred cell factory for the production of therapeutic glycoproteins. Although efforts primarily within bioprocess optimization have led to increased product titers of recombinant proteins (r-proteins) expressed in CHO cells, post-transcriptional bottlenecks in the biosynthetic pathway of r-proteins remain to be solved. To this end, the ectopic expression of transgenes (effector genes) offers great engineering potential. However, studies on effector genes have in some cases led to inconsistent results. Whereas this can in part be attributed to product specificity, other experimental and cellular factors are likely important contributors to these conflicting results. Here, these factors are reviewed and discussed with the objective of guiding future studies on effector genes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biotechnology Advances - Volume 35, Issue 1, January–February 2017, Pages 64-76
نویسندگان
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