کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6451208 1361336 2016 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research review paperSperm processing for advanced reproductive technologies: Where are we today?
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Research review paperSperm processing for advanced reproductive technologies: Where are we today?
چکیده انگلیسی

Assisted reproductive technologies (ARTs) utilize sperm sorting methods to select viable sperm from the semen samples. Conventional sperm sorting techniques in current use are density gradient centrifugation, direct swim-up, and conventional swim-up. These methods use multiple centrifugation steps, which have been shown to generate reactive oxygen species (ROS) that decrease DNA integrity and damage sperm. Newer technologies, such as microfluidics, electrophoresis, motile sperm organelle morphology examination (MSOME), and birefringence eliminate the centrifugation steps and can improve the selection of sperm with higher DNA integrity, normal morphology, and motility as well as improved artificial insemination outcomes. In this review, we discuss some recent research in centrifugation and non-centrifugation based techniques and their effect on sperm quality and ART outcomes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biotechnology Advances - Volume 34, Issue 5, September–October 2016, Pages 578-587
نویسندگان
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