One MoNomer doubly imprinted dendrimer nanofilm modified pencil graphite electrode for simultaneous electrochemical determination of norepinephrine and uric acid

- This work is novel as it involves an innovative idea of combining dual template imprinting and One MoNomer molecular imprinting for the development of a dendritic nano-film.
- The homogenously dispersed molecular cavities (dendritic-boxes), facilitating better ingress-egress of simultaneous mixture of NE and UA.
- GNPs-fMWCNTs embedded OMNiDID film served as 'nano-wire' to induce electroconductivity and thereby improved sensitivity.
- The detection limits realized by the proposed sensor, are found to be as low as 0.62 ng mL−1 for NE and 0.43 ng mL−1 for UA (S/N = 3), in aqueous, biological and pharmaceutical samples.

One MoNomer dual imprinted hyperbranched polymer (dendrimer) with dispersed gold nanoparticle-functionalized multiwalled carbon nanotubes composite was used for the growth of a nanometer thin film applying 'surface-grafting from' approach on the pencil graphite electrode. The idea of combining dual template imprinting and One MoNomer molecular imprinting for the development of a dendritic nano-film, with homogenously generated molecular cavities (dendritic-boxes), is novel for the simultaneously better ingress-egress of pair of analytes (norepinephrine and uric acid). The differential pulse anodic stripping voltammetric peak potentials for both the analytes were found to be well apart approximately by 200 mV. This enabled simultaneous analysis, one in the presence of other, without any cross reactivity, interferences, and false-positives. The detection limits realized by the proposed sensor, under optimized analytical conditions, were found to be as low as 0.62 ng mL−1 for norepinephrine and 0.43 ng mL−1 for uric acid (S/N = 3) in aqueous, biological and pharmaceutical samples. Such stringent limits could be considered suitable for the primitive diagnosis of several chronic diseases, in clinical settings.

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