کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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6477204 | 1426595 | 2016 | 8 صفحه PDF | دانلود رایگان |
- l-Cysteine determination using bulk-Co(II)-phthalocyanine nanoparticle modified disposable screen-printed graphite electrode is reported.
- CoPc-SPE is used for the first time for l-cysteine determination in embryo cell culture media.
- l-Cysteine electrochemical sensor for an indirect evaluation of oxidative stress in embryo cell culture media is possible.
Thiol-containing compounds such as l-cysteine have been demonstrated to play an important role in metabolism and cellular growth, acting as powerful antioxidants. Consequently, their analytical determination in biological media has received a considerable amount of attention. In this work, an electrochemical sensor for the accurate electroanalytical determination of l-cysteine is proposed, based upon a Co(II)-phthalocyanine bulk modified disposable screen-printed graphite electrode (CoPc-SPE). Cyclic (CV) and Square Wave (SWV) voltammetry experiments have demonstrated an excellent electrocatalytic activity towards the electrochemical oxidation of l-cysteine using CoPc-SPEs within optimum neutral or basic pH. Moreover, the SWV response of l-cysteine is found to exhibit a linear range of 2.6-200 μM, with a low limit of detection of 4 μM (S/N = 3) and a sensitivity of 0.78 μA cmâ 2 μMâ 1. Coefficient of variation for reproducibility and repeatability of l-cysteine determination using the CoPc-SPE are 3 and 0.4%, respectively. The effect of inherent interferences such as naturally occurring amino acids, cystine and cysteic acid has been also evaluated. Finally, the applicability of the l-cysteine electrochemical sensor based upon CoPc-SPEs has been successfully demonstrated for the first time for the assessment of l-cysteine in a complex embryo cell culture medium.
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Journal: Journal of Electroanalytical Chemistry - Volume 780, 1 November 2016, Pages 303-310