کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6482239 1415982 2018 32 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Combining engineering strategies and fermentation technology to enhance docosahexaenoic acid (DHA) production from an indigenous Thraustochytrium sp. BM2 strain
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Combining engineering strategies and fermentation technology to enhance docosahexaenoic acid (DHA) production from an indigenous Thraustochytrium sp. BM2 strain
چکیده انگلیسی
The DHA productivity of Thraustochytrium sp. BM2 was improved by optimizing the bioreactor operation conditions. The effects of agitation rate and aeration rate on the microalgal growth and DHA production were first studied in 5-L stirred-tank fermenter. The results showed that 100 rpm agitation and 1.2 vvm aeration (i.e., kLa = 27 ± 1.1 h−1) achieved the highest biomass (4590 mg/L/d) and DHA (379 mg/L/d) productivities. Next, using an optimal glucose concentration (60 g/L) led to the highest biomass (5580 mg/L/d) and DHA (457 mg/L/d) productivities. The cellular DHA content decreased when increasing the agitation rate, probably due to the sensitivity of this strain to the shear stress. The effect of pneumatic and mechanical agitation under the same kLa was studied with two different types of fermenters (stirred-tank and air-lift) and cultivation in air-lift fermenter resulted in better biomass production (27.57 g/L), cellular DHA content (13.1%) and DHA productivity (610 mg/L/d).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Engineering Journal - Volume 133, 15 May 2018, Pages 179-185
نویسندگان
, ,