کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6482246 1415983 2018 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Scaling of massively parallel patient-specific cell cultures with a transportable conditioned cell culture chamber
ترجمه فارسی عنوان
مقیاس سلول های سلولی اختصاصی به طور موازی موثر با یک محفظه کشت سلول های محرمانه حمل و نقل
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
Barrier Isolators, which separate the cell culture processing atmosphere from the bioburden introduced by personnel, can reduce contamination risks to a cell or tissue product. Compared to open clean room processing, isolator modules can be more readily replicated to scale-up operations. However, for processing of massively parallel patient-specific cell cultures, incubation capacity in a barrier isolator can be a bottleneck. Flexible incubation capacity can be provided by external incubators if cultures can be safely transported to and from the isolator for processing. We tested a transportable conditioned cell culture chamber (TC4) designed for this purpose. We previously published on good cell growth using this processing system to expand K562. In this study, we performed mock production runs with a highly permissive color-changing bacterial broth. We took samples of the final mock cell product and incubated them for bacterial growth. We also performed environmental monitoring of the cell processing chamber with an air sampler and contact plates. Positive control samples were all yellow and turbid. Negative samples and all test materials were negative for microbial growth. We concluded that this transport chamber could safely alleviate the bottleneck in cell production presented by the needs of massively-parallel patient specific cell incubation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Engineering Journal - Volume 132, 15 April 2018, Pages 139-144
نویسندگان
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