Optimization of Chlorella sorokiniana cultivation condition for simultaneous enhanced biomass and lipid production via CO2 fixation

In this work, the cultivation condition of Chlorella sorokiniana was investigated to optimize biomass and lipid production. The influence of initial biomass content, nitrogen sources and concentration, phosphorus concentration, pH and CO2 concentration was tested. The experimental results indicated that 1.0 g/L glycine could be a suitable nitrogen source for Chlorella sorokiniana. Meanwhile, KH2PO4 with the concentration of 11.4 mg/L at the pH of 9 was preferable phosphorus source for biomass and lipid accumulation. The maximum lipid concentration and content could achieve 173.73 mg/L and 44.1%. When CO2 was added as inorganic carbon source with the gas flow rate of 6 sccm, the maximum biomass concentration was 1.0 g/L on the fourth day.