کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6483925 1415979 2018 37 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The global regulator IrrE from Deinococcus radiodurans enhances the furfural tolerance of Saccharomyces cerevisiae
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
The global regulator IrrE from Deinococcus radiodurans enhances the furfural tolerance of Saccharomyces cerevisiae
چکیده انگلیسی
High tolerance to inhibitors is crucial for microorganisms and difficult to achieve by manipulating single genes because strain tolerance involves multiple genes and several complicated regulation mechanisms. The present study demonstrated that irrE gene from Deinococcus radiodurans R1 promoted the furfural resistance of Saccharomyces cerevisiae through directed evolution. Compared to the wild-type strain, the FR mutant not only showed improved tolerance to single- or multi- inhibitors (furfural, 5-hydroxymethylfurfural, formic and acetic acid), but also obtained better ability of eliminating reactive oxygen species. Consequently, batch fermentation of the FR mutant in synthetic medium (200 g/L glucose and 2.0 g/L furfural) resulted in an 37% increase in ethanol productivity, although ethanol titer (∼88 g/L) was comparable to that of the control. This study provides the first evidence that an exogenous global regulator can improve the stress tolerance of yeast, which is significant for production of industrial products from lignocellulose by S. cerevisiae.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Engineering Journal - Volume 136, 15 August 2018, Pages 69-77
نویسندگان
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