کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6483980 134 2016 36 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Correlation for the maximum oxygen transfer capacity in shake flasks for a wide range of operating conditions and for different culture media
ترجمه فارسی عنوان
همبستگی برای حداکثر ظرفیت انتقال اکسیژن در فلاسک لرزش برای طیف گسترده ای از شرایط عملیاتی و برای رسانه های مختلف کشت
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
Characterization of shake flasks is essential to ensure a sufficient oxygen supply for aerobic microorganism. To determine the volumetric mass transfer coefficient kLa or the maximum oxygen transfer capacity OTRmax the sulfite system is commonly applied. It has been used for non-baffled shake flasks based on the assumption of a proportionality between the kLa of the sulfite system and of a biological culture. This work proves this to be valid only for shaking frequencies above 300 rpm. Below the proportionality factor depends on the shaking frequency. More than 350 experiments with different concentrations of the sulfite system, 16 different media compositions and 10 different microorganisms were evaluated varying the shaking diameter (1.25-10 cm), flask diameter (51-131 mm), filling volume (2-160 mL) and shaking frequency (100-450 rpm). For the first time the properties of the culture media are coherently represented by a term based on the easily accessible osmolality. An universal consistent equation to calculate the OTRmax for non-baffled shake flasks for an exhaustive range of shaking conditions was developed and validated by experimental data. The deviations are within a remarkable narrow range of ±5 mmol L−1 h−1.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Engineering Journal - Volume 109, 15 May 2016, Pages 228-235
نویسندگان
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