کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6486165 | 420 | 2015 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Matrix stiffness determines the fate of nucleus pulposus-derived stem cells
ترجمه فارسی عنوان
سختی ماتریس تعیین سرنوشت هسته سلول های بنیادی پالوسوس را تعیین می کند
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کلمات کلیدی
قابلیت ارتجاعی، سختی ماتریس، فیبرینوژن، هیدروژل، سلول های بنیادی، دیسک بین مهره ای،
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
Intervertebral disc (IVD) degeneration and consequent low-back pain present a major medical challenge. Nucleus pulposus-derived stem cells (NP-SCs) may lead to a novel therapy for this severe disease. It was recently shown that survival and function of mature NP cells are regulated in part by tissue stiffness. We hypothesized that modification of matrix stiffness will influence the ability of cultured NP-SCs to proliferate, survive, and differentiate into mature NP cells. NP-SCs were subcultured in three-dimensional matrices of varying degrees of stiffness as measured by the material's shear storage modulus. Cell survival, activity, and rate of differentiation toward the chondrogenic or osteogenic lineage were analyzed. NP-SCs were found to proliferate and differentiate in all matrices, irrespective of matrix stiffness. However, matrices with a low shear storage modulus (Gâ²Â = 1 kPa) promoted significantly more proliferation and chondrogenic differentiation, whereas matrices with a high modulus (Gâ²Â = 2 kPa) promoted osteogenic differentiation. Imaging performed via confocal and scanning electron microscopes validated cell survival and highlighted stiffness-dependent cell-matrix interactions. These results underscore the effect of the matrix modulus on the fate of NP-SCs. This research may facilitate elucidation of the complex cross-talk between NP-SCs and their surrounding matrix in healthy as well as pathological conditions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 49, May 2015, Pages 68-76
Journal: Biomaterials - Volume 49, May 2015, Pages 68-76
نویسندگان
Yosi Navaro, Nadav Bleich-Kimelman, Lena Hazanov, Iris Mironi-Harpaz, Yonatan Shachaf, Shai Garty, Yoav Smith, Gadi Pelled, Dan Gazit, Dror Seliktar, Zulma Gazit,