Unintended consequences? Water molecules at biological and crystallographic protein-protein interfaces

- Waters play similar roles at biological and non-biological protein-protein interfaces.
- Biological interfaces are more hydrophobic but have better organized hydrogen bonding.
- Biological interfaces more frequently water-mediate interactions with backbone atoms.
- Aromatic/planar residues are more observed in biological water-mediated interactions.
- Because of its charge and sweep volume lysine opportunistically forms interactions.