کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6488282 | 42631 | 2015 | 32 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Codon optimization, promoter and expression system selection that achieved high-level production of Yarrowia lipolytica lipase in Pichia pastoris
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Codon optimization, promoter and expression system selection that achieved high-level production of Yarrowia lipolytica lipase in Pichia pastoris Codon optimization, promoter and expression system selection that achieved high-level production of Yarrowia lipolytica lipase in Pichia pastoris](/preview/png/6488282.png)
چکیده انگلیسی
- The codons of Y. lipolytica lipase gene were optimized by de novo gene design and synthesis.
- Codon optimized lipase gene has significantly higher expression level than the native gene.
- PAOX1 controlled lipase gene has higher expression level than PFLD1 controlled.
- Lipase gene in Pichia GS115 has equal activity than newly developed PichiaPink system.
- Lipase activity of codon optimized recombinants in fermenter reached 38,500Â U/mL.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 71, April 2015, Pages 66-72
Journal: Enzyme and Microbial Technology - Volume 71, April 2015, Pages 66-72
نویسندگان
Wen-Jing Zhou, Jiang-Ke Yang, Lin Mao, Li-Hong Miao,