کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6489999 | 1416546 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
New nucleoside hydrolase with transribosylation activity from Agromyces sp. MM-1 and its application for enzymatic synthesis of 2â²-O-methylribonucleosides
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
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چکیده انگلیسی
Microorganisms were screened for transribosylation activity between 2â²-O-methyluridine (2â²-OMe-UR) and nucleobases, for the purpose of developing a biotransformation process to synthesize 2â²-O-methylribonucleosides (2â²-OMe-NRs), which are raw materials for nucleic acid drugs. An actinomycete, Agromyces sp. MM-1 was found to produce 2â²-O-methyladenosine (2â²-OMe-AR) when whole cells were used in a reaction mixture containing 2â²-OMe-UR and adenine. The enzyme responsible for the transribosylation was partially purified from Agromyces sp. MM-1Â cells through a six-step separation procedure, and identified as a nucleoside hydrolase family enzyme termed AgNH. AgNH was a bi-functional enzyme catalyzing both hydrolysis towards 2â²-OMe-NRs and transribosylation between 2â²-OMe-UR and various nucleobases as well as adenine. In the hydrolysis reaction, AgNH preferred guanosine analogues as its substrates. In the transribosylation reaction, AgNH showed strong activity towards 6-chloroguanine, with 25-fold relative activity when adenine was used as the acceptor substrate. The transribosylation reaction product from 2â²-OMe-UR and 6-chloroguanine was determined to 2â²-O-methyl-6-chloroguanosine (2â²-OMe-6ClGR). Under the optimal conditions, the maximum molar yield of 2â²-OMe-6ClGR reached 2.3% in a 293-h reaction, corresponding to 440Â mg/L.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Bioscience and Bioengineering - Volume 125, Issue 1, January 2018, Pages 38-45
Journal: Journal of Bioscience and Bioengineering - Volume 125, Issue 1, January 2018, Pages 38-45
نویسندگان
Yuuki Mitsukawa, Makoto Hibi, Narihiro Matsutani, Nobuyuki Horinouchi, Satomi Takahashi, Jun Ogawa,