کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6490047 | 1416546 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Arabidopsis thaliana cold-regulated 47 gene 5â²-untranslated region enables stable high-level expression of transgenes
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Transgene expression is regulated through several steps, this study focuses on the mRNA translation step. The expression level of transgenes can be increased by 5â²-untranslated region (5â²UTR) sequences in certain genes which act as translational enhancers. On the other hand, translation in most mRNA species is repressed by growth, development, and stress events. There is a possibility that transgene mRNA is also repressed in these conditions, despite the use of a translational enhancer. Therefore, a consistently efficient translational enhancer is needed to develop a reliable transgene expression system. Herein we searched for mRNAs translated stably under different growth, development and environmental conditions using data sets of polysome fraction assays and microarray analysis. Correct 5â²UTR sequences of candidate genes were determined by cap analysis of gene expression and we tested translational ability of the candidate 5â²UTRs by reporter assays. We found the 5â²UTR of cold-regulated 47 gene to be an effective translational enhancer, contributing to stable high-level expression under various conditions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Bioscience and Bioengineering - Volume 125, Issue 1, January 2018, Pages 124-130
Journal: Journal of Bioscience and Bioengineering - Volume 125, Issue 1, January 2018, Pages 124-130
نویسندگان
Shotaro Yamasaki, Yuji Sanada, Ryoji Imase, Hideyuki Matsuura, Daishin Ueno, Taku Demura, Ko Kato,