کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6490047 | 1416546 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Arabidopsis thaliana cold-regulated 47 gene 5â²-untranslated region enables stable high-level expression of transgenes
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Arabidopsis thaliana cold-regulated 47 gene 5â²-untranslated region enables stable high-level expression of transgenes Arabidopsis thaliana cold-regulated 47 gene 5â²-untranslated region enables stable high-level expression of transgenes](/preview/png/6490047.png)
چکیده انگلیسی
Transgene expression is regulated through several steps, this study focuses on the mRNA translation step. The expression level of transgenes can be increased by 5â²-untranslated region (5â²UTR) sequences in certain genes which act as translational enhancers. On the other hand, translation in most mRNA species is repressed by growth, development, and stress events. There is a possibility that transgene mRNA is also repressed in these conditions, despite the use of a translational enhancer. Therefore, a consistently efficient translational enhancer is needed to develop a reliable transgene expression system. Herein we searched for mRNAs translated stably under different growth, development and environmental conditions using data sets of polysome fraction assays and microarray analysis. Correct 5â²UTR sequences of candidate genes were determined by cap analysis of gene expression and we tested translational ability of the candidate 5â²UTRs by reporter assays. We found the 5â²UTR of cold-regulated 47 gene to be an effective translational enhancer, contributing to stable high-level expression under various conditions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Bioscience and Bioengineering - Volume 125, Issue 1, January 2018, Pages 124-130
Journal: Journal of Bioscience and Bioengineering - Volume 125, Issue 1, January 2018, Pages 124-130
نویسندگان
Shotaro Yamasaki, Yuji Sanada, Ryoji Imase, Hideyuki Matsuura, Daishin Ueno, Taku Demura, Ko Kato,