کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6493090 | 44040 | 2011 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Water-soluble phosphorus porphyrins with high activity for visible light-assisted inactivation of Saccharomyces cerevisiae
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In an effort to develop water-soluble singlet-oxygen (1O2) photosensitizer, we synthesized a series of phosphorus porphyrin complexes (1, (RO)2P(tpp)+Clâ, tpp = tetraphenylporphyrinato ligand, R = -(CH2CH2O)m-(CH2)n-H, m = 0-3, n = 1, 2, 4, 6) and evaluated their performance characteristics. Water-solubilities of 1 (m = 1, 2 and n = 1, 2, 4, 6) were determined to be >1.0 mM, which was higher than the parent (HO)2P(tpp)+Clâ. The quantum yields for the generation of 1O2 in water based on the amount of 11* were determined to be 0.73, 0.69, and 0.62 for 1d (m = 2, n = 4), 1e (m = 2, n = 2), and 1f (m = 2, n = 1), respectively. Photo-inactivation by 1 was performed with yeast (Saccharomyces cerevisiae), and was evaluated using a inactivation factor AF = ([1]M Ã T1/2)â1, where [1]M is the minimum effective concentration of 1 and T1/2 is a half-life of the yeast initially used. In the case of 1b (m = 1, n = 6) and 1c (m = 2, n = 6), [1]M was determined to be 5 nM in the photo-sterilization. Bio-affinity was evaluated by the adsorbed concentration of 1 ([P]ad) into the yeast, which was measured by absorption spectrophotometry using a confocal laser-scanning microscope. These findings revealed that a large amount of 1 was adsorbed into the yeast. The estimated amount of 1b obtained from 10 μM aqueous solution came to 171 mM (17,100 fold) in terms of concentration. As an increase in carbon number (n) of the alkyl group on axial ligands, AF and [P]ad increased. It is strongly suggested that the photo-inactivation took place with the assistance of the attack of 1O2 inside the cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 218, Issue 1, 5 February 2011, Pages 178-184
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 218, Issue 1, 5 February 2011, Pages 178-184
نویسندگان
Jin Matsumoto, Tomohiko Shinbara, Shin-ichiro Tanimura, Tomoko Matsumoto, Tsutomu Shiragami, Haruhiko Yokoi, Yoshio Nosaka, Shigetoshi Okazaki, Kazutaka Hirakawa, Masahide Yasuda,