کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6494225 | 44798 | 2016 | 18 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Reaction kinetic analysis of the 3-hydroxypropionate/4-hydroxybutyrate CO2 fixation cycle in extremely thermoacidophilic archaea
ترجمه فارسی عنوان
تجزیه و تحلیل سینتیکی واکنش سیلیکون 3-هیدروکسی پروپیونات / 4-هیدروکسی بوتییدرات در چرخه احیاء بسیار گرمایی
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کلمات کلیدی
MCMHBCSMetallosphaera sedulaHPCDMethylmalonyl-CoA mutaseMCEACCACRHBCDAACT4-hydroxybutyrateMCRMSRSSR3-hydroxypropionate - 3-هیدروکسی پروپیوناتHPCs - HPC هاSuccinic semialdehyde dehydrogenase - semialdehydedehydrogenase سسنزینCO2 fixation - تثبیت CO2Succinic semialdehyde reductase - سولفات سمیالیدیدید ردوکتازSSADH - علامتInorganic pyrophosphatase - پریففسفاتاز معدنی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
The 3-hydroxypropionate/4-hydroxybutyrate (3HP/4HB) cycle fixes CO2 in extremely thermoacidophilic archaea and holds promise for metabolic engineering because of its thermostability and potentially rapid pathway kinetics. A reaction kinetics model was developed to examine the biological and biotechnological attributes of the 3HP/4HB cycle as it operates in Metallosphaera sedula, based on previous information as well as on kinetic parameters determined here for recombinant versions of five of the cycle enzymes (malonyl-CoA/succinyl-CoA reductase, 3-hydroxypropionyl-CoA synthetase, 3-hydroxypropionyl-CoA dehydratase, acryloyl-CoA reductase, and succinic semialdehyde reductase). The model correctly predicted previously observed features of the cycle: the 35-65% split of carbon flux through the acetyl-CoA and succinate branches, the high abundance and relative ratio of acetyl-CoA/propionyl-CoA carboxylase (ACC) and MCR, and the significance of ACC and hydroxybutyryl-CoA synthetase (HBCS) as regulated control points for the cycle. The model was then used to assess metabolic engineering strategies for incorporating CO2 into chemical intermediates and products of biotechnological importance: acetyl-CoA, succinate, and 3-hydroxypropionate.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Metabolic Engineering - Volume 38, November 2016, Pages 446-463
Journal: Metabolic Engineering - Volume 38, November 2016, Pages 446-463
نویسندگان
Andrew J. Loder, Yejun Han, Aaron B. Hawkins, Hong Lian, Gina L. Lipscomb, Gerrit J. Schut, Matthew W. Keller, Michael W.W. Adams, Robert M. Kelly,