کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6494403 | 44804 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Engineering l-arabinose metabolism in triacylglycerol-producing Rhodococcus opacus for lignocellulosic fuel production
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
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چکیده انگلیسی
Advanced biofuels from lignocellulosic biomass have been considered as a potential solution for the issues of energy sustainability and environmental protection. Triacylglycerols (TAGs) are potential precursors for the production of lipid-based liquid biofuels. Rhodococcus opacus PD630 can accumulate large amounts of TAGs when grown under physiological conditions of high carbon and low nitrogen. However, R. opacus PD630 does not utilize the sugar l-arabinose present in lignocellulosic hydrolysates. Here, we report the engineering of R. opacus to produce TAGs on l-arabinose. We constructed a plasmid (pASC8057) harboring araB, araD and araA genes derived from a Streptomyces bacterium, and introduced the genes into R. opacus PD630. One of the engineered strains, MITAE-348, was capable of growing on high concentrations (up to 100Â g/L) of l-arabinose. MITAE-348 was grown in a defined medium containing 16Â g/L l-arabinose or a mixture of 8Â g/L l-arabinose and 8Â g/L d-glucose. In a stationary phase occurring 3 days post-inoculation, the strain was able to completely utilize the sugar, and yielded 2.0Â g/L for l-arabinose and 2.2Â g/L for l-arabinose/d-glucose of TAGs, corresponding to 39.7% or 42.0%, respectively, of the cell dry weight.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Metabolic Engineering - Volume 30, July 2015, Pages 89-95
Journal: Metabolic Engineering - Volume 30, July 2015, Pages 89-95
نویسندگان
Kazuhiko Kurosawa, Jens Plassmeier, Jörn Kalinowski, Christian Rückert, Anthony J. Sinskey,