کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6494403 44804 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Engineering l-arabinose metabolism in triacylglycerol-producing Rhodococcus opacus for lignocellulosic fuel production
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Engineering l-arabinose metabolism in triacylglycerol-producing Rhodococcus opacus for lignocellulosic fuel production
چکیده انگلیسی
Advanced biofuels from lignocellulosic biomass have been considered as a potential solution for the issues of energy sustainability and environmental protection. Triacylglycerols (TAGs) are potential precursors for the production of lipid-based liquid biofuels. Rhodococcus opacus PD630 can accumulate large amounts of TAGs when grown under physiological conditions of high carbon and low nitrogen. However, R. opacus PD630 does not utilize the sugar l-arabinose present in lignocellulosic hydrolysates. Here, we report the engineering of R. opacus to produce TAGs on l-arabinose. We constructed a plasmid (pASC8057) harboring araB, araD and araA genes derived from a Streptomyces bacterium, and introduced the genes into R. opacus PD630. One of the engineered strains, MITAE-348, was capable of growing on high concentrations (up to 100 g/L) of l-arabinose. MITAE-348 was grown in a defined medium containing 16 g/L l-arabinose or a mixture of 8 g/L l-arabinose and 8 g/L d-glucose. In a stationary phase occurring 3 days post-inoculation, the strain was able to completely utilize the sugar, and yielded 2.0 g/L for l-arabinose and 2.2 g/L for l-arabinose/d-glucose of TAGs, corresponding to 39.7% or 42.0%, respectively, of the cell dry weight.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Metabolic Engineering - Volume 30, July 2015, Pages 89-95
نویسندگان
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