Rapid determination of benzodiazepines, zolpidem and their metabolites in urine using direct injection liquid chromatography-tandem mass spectrometry

- A rapid and simple LC-MS/MS method was developed for benzodiazepines, zolpidem and their metabolites.
- An aliquot (5 μL) of the sample solution was directly injected into the LC-MS/MS.
- The separation and detection of 18 analytes were achieved within 10 min with good precision and low detection limits.
- The applicability of this method was successfully verified to human urine samples from drug users.