کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6584657 456358 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Electrochemical recycling of gold nanofibrous membrane as an enzyme immobilizing carrier
ترجمه فارسی عنوان
بازیافت الکتروشیمیایی غشای نانویی فیبر طلا به عنوان یک حامل بی حرکت کننده آنزیم
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
چکیده انگلیسی
A gold nanofibrous membrane was shown to be an enzyme-immobilizing carrier that can be recycled on-site in a continuous flow-through reactor using a simple electrochemical treatment. The membrane was prepared by electrospinning a polyacrylonitrile/gold salt solution, followed by electroless gold plating. Laccase, used as a model enzyme, was immobilized on the gold membrane surface by a combination of formation of amino-terminated self-assembled monolayers and glutaraldehyde treatment. Enzyme desorption at neutral pH was confirmed by a 98% decrease in enzymatic activity, based on oxidation of a 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) substrate, after electrochemical treatment for desorption, and surface characterization using X-ray photoelectron spectroscopy. The enzymatic activity after re-immobilization on the membrane was restored to 90%, and decreased to 1.0% after redesorption. These results showed that recycling of the gold nanofibrous membrane as an enzyme-immobilizing carrier was possible. The laccase-immobilized membrane was used in a flow-through reactor; the activities of the immobilized and re-immobilized enzymes on the membrane were 62.7% and 58.5% of the respective initial activities after operation for 7 d.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemical Engineering Journal - Volume 280, 15 November 2015, Pages 558-563
نویسندگان
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