کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6594896 1423732 2018 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Modelling the effect of temperature on the gel-filtration chromatographic protein separation
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Modelling the effect of temperature on the gel-filtration chromatographic protein separation
چکیده انگلیسی
In this study a mechanistic model for chromatography was taken and the solution to its Laplace transfer function was obtained using the Fast Fourier Transform method. Using previously developed correlations for modelling diffusion, both in solution and intraparticle, and estimating the mass transfer coefficient, the effect of temperature in gel fittration liquid chromatography was investigated. The effect of each individual parameter on the elution curve was systematically explored allowing for reasonable estimates for the different temperature cases. In this case, a system of bovine serum albumin and phenylalanine separated by gel filtration chromatography was simulated to demonstrate how the resolution and the selectivity of the separation will change with physical parameters. Decreasing the particle size and flow rate while increasing the temperature led to higher resolution, which is consistent with experimental literature data.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Computers & Chemical Engineering - Volume 112, 6 April 2018, Pages 304-315
نویسندگان
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