کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6602785 1424080 2018 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Construction and characterization of flavin adenine dinucleotide glucose dehydrogenase complex harboring a truncated electron transfer subunit
ترجمه فارسی عنوان
ساخت و مشخص کردن ترکیب گلوکز دی هیدروژناز آدنین دینوکوتیید فلاوین با استفاده از یک زیر واحد انتقال الکترون کوتاه
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
چکیده انگلیسی
FADGDH complex harboring a truncated electron transfer subunit composed of only heme 3 was constructed. A truncated electron transfer subunit thus, the designed trβ subunit was expressed as a soluble and functional heme c molecule and formed a complex with a γα catalytic complex. The formed FADGDH complex showed inter-molecular electron transfer from the FAD to the trβ subunit and from the trβ subunit to an external electron acceptor and showed electron transfer subunit-mediated characteristic dye-mediated dehydrogenase activity with a Ru complex. Moreover, the FADGDH complex harboring the trβ subunit showed DET activity toward the electrode. Spectroelectrochemical observations revealed that the trβ subunit possessed a lower formal potential than that of any of the 3 hemes existing in the whole electron transfer subunit.262
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Electrochimica Acta - Volume 277, 1 July 2018, Pages 276-286
نویسندگان
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