Non-Fluorescence label protein sensing with track-etched nanopore decorated by avidin/biotin system

Single nanopore technology permits to design a biosensor, able to detect proteins without the use of specific probe. In this work, we have fashioned cylindrical nanopore by track-etched technique and atomic layer deposition. The nanopore was then decorated by the asymmetric tethering of biotin. From the rectification of the I-V response induced by the asymmetry of the nanopore, we demonstrate the potentiality of this strategy to detect and to discriminate avidin and streptavidin with respect to their global charge. Based on the nanopore decorated with avidin and streptavidin, we show the possible detection layer by layer of biotinylated protein (IgG and BSA) as well as antibody.