کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6607066 459524 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Bioelectrochemical characterization of the reconstruction of heterotrimeric fructose dehydrogenase from its subunits
ترجمه فارسی عنوان
خصوصیات بیوالکترو شیمیایی بازسازی فروکتوز دهیدروژناز هتروترومرییک از زیرمجموعه های آن
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
چکیده انگلیسی
d-Fructose dehydrogenase (FDH) is a heterotrimeric-membrane-bound enzyme capable of direct electron transfer (DET)-type bioelectrocatalysis. Subunit II contains three heme C moieties and is presumed to play a key role in the DET reaction because the subunit I/III subcomplex (without subunit II) lacks DET-type activity. We constructed an expression system for subunit II. A non-turnover signal from subunit II was not observed on voltammograms, and a subunit II-adsorbed electrode did not exhibit DET-type activity in the presence of the subunit I/III subcomplex and fructose. Gel filtration column chromatography indicated that subunit II formed multimeric complexes with four or more subunits. The aggregation of subunit II seemed to interfere with its direct communication with the electrodes. In contrast, when subunit II was mixed with the subunit I/III complex in a solution, they formed a 1:1 full complex, and the complex recovered DET-type bioelectrocatalytic activity. These results strongly suggest that the heme C in subunit II is the electron transfer site for the DET-type bioelectrocatalytic activity of FDH.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Electrochimica Acta - Volume 210, 20 August 2016, Pages 689-694
نویسندگان
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