کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6613664 | 459600 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A sandwich-type label-free electrochemiluminescence immunosensor for neurotensin based on sombrero model with graphene-hyaluronate-luminol composite
ترجمه فارسی عنوان
یک ایمونوسنسور الکتروشیمیومینسانس الکتروشیمیایی ساندویچ برای نورونزن بر اساس مدل سمبروه با کامپوزیت گرافن هیالورونات لومینول
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
مهندسی شیمی (عمومی)
چکیده انگلیسی
An ultrasensitive sandwich-type label-free electrochemiluminescence (ECL) immunosensor based on sombrero model for the detection of neurotensin (NT) in human serum and urine was developed. Graphene-hyaluronate-luminol (G-HY-luminol) composite was coated on the surface of the glassy carbon electrode to build the ECL immunosensor, which gave in-situ ECL signals by luminol. A secondary NT unlabelled antibody was used to form the final sandwich immunocomplex as a sombrero model, facilitating reduction of the ECL intensity to improve the sensitivity of detection significantly. Results showed that the inhibition of ECL intensity increased with the logarithm of NT concentration within a wide linear range from 0.001 pg cmâ3 to 100 pg cmâ3. In addition, specificity, stability, reproducibility, regeneration and application were satisfactory. Therefore, this developed ECL immunosensor has a potential for practical detection of NT and NT-like peptides with small molecular weight in the clinical diagnostics.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Electrochimica Acta - Volume 135, 20 July 2014, Pages 519-525
Journal: Electrochimica Acta - Volume 135, 20 July 2014, Pages 519-525
نویسندگان
Zhiyong Guo, Shuping Du, Beibei Chen, Yuhong Sha, Bin Qiu, Xiaohua Jiang, Sui Wang, Xing Li,