Electrochemistry and UV-vis spectroscopy of synthetic thiocholine: Revisiting the electro-oxidation mechanism

- The results imply comprehension in biosensors for pesticides detection which employ acetylcholinesterase and detect thiocholine.
- The anodic oxidation mechanism of thiocholine was elucidated for the first time (dimer and the number of electrons).
- The thiocholine electrolysis demonstrated that the number of electrons involved in the process is two.
- Reaction between dithiothreitol and electrolysed thiocholine identified the dimer.
- Raman spectroscopy evidenced the dimer formation due to the S-H peak extinction.