Proteomic analysis of latex from Jatropha curcas L. stems and comparison of two classic proteomic sample isolation methods: The phenol extraction and the TCA/acetone extraction

A large number of proteins were found and exclusively identified by either method, indicating that a better proteome coverage of plant samples in a similar context needs the combined use of multiple isolation methods. In addition, the core biological function of the latex may be uncovered by certain GO, COG, and KEGG classes/categories/pathways that annotate more proteins.