کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6661889 | 1426559 | 2018 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Electrochemical detection of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone using a cytochrome P450 2E1 decorated biosensor
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In this work, a novel electrochemical biosensor for 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) was developed based on cytochrome P450 2E1 (CYP2E1). The biosensor was fabricated by immobilization of CYP2E1 with poly (diallyldimethylammonium chloride) (PDDA) - poly (sodium-p-styrenesulfonate) (PSS) electrolyte film on a screen-printed carbon electrode (SPCE) electrodeposited with gold nanoparticles (AuNPs). The procedures of electrode modification were characterized by using scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). With the good conductivity of AuNPs as well as the immobilized CYP2E1, the electrochemical performances of the proposed biosensor for the detection of NNK showed a wide linear range of 0-386â¯Î¼M with a sensitivity of 79â¯Î¼Aâ¯cmâ2â¯mMâ1 and a low detection limit of 7.71â¯Î¼M. Additionally, excellent selectivity and reproducibility were also obtained. The fabricated NNK electrochemical biosensor was successfully employed in urine sample determination, exhibiting great potential in the real sample analysis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Electroanalytical Chemistry - Volume 816, 1 May 2018, Pages 62-67
Journal: Journal of Electroanalytical Chemistry - Volume 816, 1 May 2018, Pages 62-67
نویسندگان
Hongli Zhao, Haiyan He, Libo Shi, Xuan Cai, Hong Li, Minbo Lan, Qian Zhang,