کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6662080 1426563 2018 27 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Developing ethanol bioanodes using a hydrophobically modified linear polyethylenimine hydrogel for immobilizing an enzyme cascade
ترجمه فارسی عنوان
تولید بیوانادهای اتانول با استفاده از یک پلی اتیلنیمین هیدروژل خطی اصلاح شده با استفاده از هیدروفوبیک برای غیر فعال شدن یک آبشار آنزیمی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
چکیده انگلیسی
Considering the importance of immobilizing dehydrogenase enzymes for bioelectronics, the focus of this paper is to obtain an enzymes cascade for ethanol bioelectrooxidation onto a carbon paper support using a hydrophobically-modified linear polymer of ethyleneimine (LPEI) as the immobilization matrix along with ethylene glycol diglycidyl ether (EGDGE) as the cross-linking agent. In the first stage of ethanol bioelectrooxidation, the biofuel cell tests show that the amount of the cross-linking agent employed in bioanode preparation directly affects their overall bioelectrocatalytic performance. In fact, much higher performance is observed for the bioanode prepared with an intermediate amount of cross-linking agent, reaching maximum current and power densities of respectively 12.5 ± 1.4 μW cm− 2 and 150 ± 17 μA cm− 2. In both the second and third steps of the ethanol oxidation cascade, several optimizations had to be performed in order to obtain a stable and functioning bioanode, but this work shows that the optimal crosslinking conditions are enzyme dependent. Although immobilization methodologies such as hydrogels have a wide application range in bioelectronics, the proportion between LPEI, EGDGE, and the enzymes being immobilized is quite important to obtain a functioning bioanode, particularly when a cascade of enzymes needs to be immobilized into this matrix.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Electroanalytical Chemistry - Volume 812, 1 March 2018, Pages 153-158
نویسندگان
, , ,