کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6662143 | 1426566 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Highly sensitive immunosensor for Hepatitis B surface antigen detection based on a novel signal amplification system of gold nanorods and mesoporous Au@Pd@Pt core-shell nanospheres
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In this work, a highly sensitive sandwich-type electrochemical immunosensor was fabricated by using the gold nanorods (Au NRs) as the substrate material and the mesoporous Au@Pd@Pt core-shell nanospheres (m-Au@Pd@Pt) as the label of the detection antibodies (Ab2, Ab2 label) for detecting Hepatitis B surface antigen (HBs Ag). The Au NRs with excellent conductivity and biocompatibility can accelerate the electron transfer on the electrode interface and enhance the load capacity of capture antibodies (Ab1). The m-Au@Pd@Pt with mesoporous structure can not only provide high specific surface area and abundant catalytically active sites, but also offer great pore accessibility of guest species from outside. The detection signal of the immunosensor was effectively improved by the novel Ab2 label. With the good cooperation between Au NRs and Ab2 label, a linear relationship between current signal and the logarithm of the HBs Ag concentration was obtained in the wide range of 20Â fg/mL to 200Â ng/mL and the detection limit of HBs Ag was 6.7Â fg/mL (signal-to-noise ratio of 3). Besides, the designed immunosensor shows good reproducibility, long-term stability and high specificity. Crucially, the sensitive and selective response to HBs Ag in real human serum samples confirms that the designed immunosensor will be promising in the clinical diagnosis of biomarkers.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Electroanalytical Chemistry - Volume 809, 15 January 2018, Pages 14-21
Journal: Journal of Electroanalytical Chemistry - Volume 809, 15 January 2018, Pages 14-21
نویسندگان
Mingdang Li, Ping Wang, Fubin Pei, Haoxuan Yu, Peng Chen, Yunhui Dong, Yueyun Li, Qing Liu, Degang Li,