کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6662437 | 463197 | 2015 | 29 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Fabrication of an amperometric urea biosensor using urease and metal catalysts immobilized by a polyion complex
ترجمه فارسی عنوان
تولید بیوسنسور اوره آمپرومتریک با استفاده از کاتالیزورهای اوره و فلز متوقف شده توسط یک پلی یون پیچیده
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کلمات کلیدی
سنسور اوره اوره، کاتالیزور فلزی، نانولوله های کربنی، پلی یون پیچیده،
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
مهندسی شیمی (عمومی)
چکیده انگلیسی
An amperometric urea biosensor based on urease and metal catalysts (Pt and Rh) has been synthesized on a multiwalled carbon nanotube (MWCNT)-modified ITO glass. A sequential coating of the metal catalysts and urease over the surface of the MWCNT-modified ITO glass was adopted for the electrode preparation by entrapment with a polyion complex (PIC). The activity of the immobilized enzyme for urea hydrolysis and the metal catalysts for the electrochemical oxidation of the ammonia generated during the enzymatic reaction were investigated. The electrochemical performance of the bioelectrode was characterized by a cyclic voltammetry technique. The sensor exhibited a linear amperometric response to urea concentration ranging from 0.05 mM to 20 mM, with a linear correlation coefficient of 0.995. A sensitivity of 1.7 μA mMâ1 cmâ2 and a response time of approximately 10 s were achieved for the hybrid electrode. The biosensor retained half of its initial activity after 20 days of storage under ambient conditions. The urea sensor employing an enzyme and metal catalysts exhibited excellent electroactivity and sensor efficiency.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Electroanalytical Chemistry - Volume 747, 15 June 2015, Pages 143-148
Journal: Journal of Electroanalytical Chemistry - Volume 747, 15 June 2015, Pages 143-148
نویسندگان
Wu Hao, Gautam Das, Hyon Hee Yoon,