A vitamin C electrochemical biosensor based on one-step immobilization of ascorbate oxidase in the biocompatible conducting poly(3,4-ethylenedioxythiophene)-lauroylsarcosinate film for agricultural application in crops

► The biocompatible and low-toxic PEDOT was improved by amino acid-based surfactant. ► The VC biosensor based on PEDOT matrix was one-step electrochemically fabricated. ► The biosensor exhibited good bioelectrocatalytic performance to VC oxidation. ► The working mechanism of the as-fabricated biosensor was proposed. ► VC levels in commercial juices and vegetable crops were detected by presented method.