Quantitative risk assessments of the effect of an edible defatted soybean meal-based antimicrobial film on the survival of Salmonella on ham

An edible antimicrobial film was prepared with defatted soybean meal (DSM) and the lactoperoxidase system (LPOS) (LPOS-DSM films) by heat pressing a film-forming mixture at 90 °C and 40 MPa for 3 min. The film was applied to ham slices and the concentration of hypothiocyanite (OSCN−) was monitored over time. Based on the data obtained, a model was developed that predicted that a film containing OSCN− at 0.66 mg/g could provide 42.9 h of protection against 2.8 log CFU/g of Salmonella Typhimurium. A Monte Carlo simulation-based analysis estimated that coating ham with the LPOS-DSM film would decrease the probability of occurrence of >1 case of salmonellosis per year by 90.7%, associated with consumption of ham. The methods of quantitative risk assessments developed in this study may also be useful for evaluating other edible antimicrobial films.