کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6765428 | 512436 | 2016 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Biosynthesis of β-caryophyllene, a novel terpene-based high-density biofuel precursor, using engineered Escherichia coli
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کلمات کلیدی
GPPMVA pathway1-deoxy-D-xylulose 5-phosphateβ-CaryophylleneIPPDXPDMAPPIPTGMevalonateFPPEscherichia coli - اشریشیا کُلیisopropyl β-d-thiogalactoside - ایزوپروپیل β-d-thiogalactosideisopentenyl pyrophosphate - ایزوپنتنیل پیرو فسفاتdimethylallyl pyrophosphate - دی متیلللی پیرو فسفاتfarnesyl diphosphate - فارسیل دی فسفاتMVA - مالیات بر ارزش افزودهpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازGeranyl diphosphate - ژانایل دی فسفاتGas chromatography - کروماتوگرافی گازی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی انرژی
انرژی های تجدید پذیر، توسعه پایدار و محیط زیست
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چکیده انگلیسی
β-caryophyllene is a common sesquiterpene compound currently being studied as a promising precursor for the production of high-density fuels. Acute demand for high-density fuels has provided the impetus to pursue biosynthetic methods to produce β-caryophyllene from reproducible sources. In this study, we produced β-caryophyllene by assembling a biosynthetic pathway in an engineered Escherichia coli strain of which phosphoglucose isomerase gene has been deleted. The 1- deoxy-d-xylulose 5-phosphate (DXP) or heterologous mevalonate (MVA) pathways were employed. Meanwhile, geranyl diphosphate synthase, glucose-6-phosphate dehydrogenase and β-caryophyllene synthase genes were co-overexpressed in the above strain. The final genetically modified strain, YJM59, produced 220 ± 6 mg/L of β-caryophyllene in flask culture. We also evaluated the use of fed-batch fermentation for the production of β-caryophyllene. After induction for 60 h, the YJM59 strain produced β-caryophyllene at a concentration of 1520 mg/L. The volumetric production fermented in the aerobic fed-batch was 0.34 mg/(L·h·OD600) and the conversion efficiency of glucose to β-caryophyllene (gram to gram) was 1.69%. Our results are the first successful attempt to produce β-caryophyllene using E. coli BL21(DE3), and provide a new strategy that is green and sustainable for the production of β-caryophyllene.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Renewable Energy - Volume 99, December 2016, Pages 216-223
Journal: Renewable Energy - Volume 99, December 2016, Pages 216-223
نویسندگان
Jianming Yang, Zhengfeng Li, Lizhong Guo, Juan Du, Hyeun-Jong Bae,