کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
679386 1459942 2016 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Introduction of the exogenous NADH coenzyme regeneration system and its influence on intracellular metabolic flux of Paenibacillus polymyxa
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
Introduction of the exogenous NADH coenzyme regeneration system and its influence on intracellular metabolic flux of Paenibacillus polymyxa
چکیده انگلیسی
The NAD+-dependent formate dehydrogenase (FDH) gene from Candida boidinii was introduced into Paenibacillus polymyxa ZJ-9. The effects of this exogenous gene on the growth of the recombinant strain P. polymyxa XG-1, FDH activity, intracellular NADH and NAD+ level and the synthesis of R,R-2,3-butanediol (R,R-2,3-BD) were determined. Results from the fermentation in the 7.5 L bioreactor showed that the exogenous FDH was highly expressed in the recombinant strain. The titers of NADH, lactic acid, ethanol, NADH/NAD+, and CO2 excretion rate (CER) of the recombinant strain increased considerably, while acetoin and formic acid decreased significantly. The highest titers of R,R-2,3-BD by the recombinant strain in batch and fed-batch fermentation were 36.8 g/L and 51.3 g/L, increased 10.2% and 8.0% compared with the parent strain, respectively. This study confirmed that coenzyme regeneration system can manipulate substance metabolism in bacteria, and is an efficient way for promoting the synthesis of NADH-dependent products.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 201, February 2016, Pages 319-328
نویسندگان
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