کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
679511 | 1459948 | 2015 | 9 صفحه PDF | دانلود رایگان |
• Neutral red-mediated electrosynthesis was applied to three different bacteria.
• Electrosynthesis altered metabolite profiles in E. coli and K. pneumoniae.
• No effect was observed on Z. mobilis metabolites.
• Hypothesized NAD+ reduction did not account for metabolite changes.
• Effects of electrosynthesis are consistent with a regulatory response.
The aim of this work was to compare the effects of electrosynthesis on different bacterial species. The effects of neutral red-mediated electrosynthesis on the metabolite profiles of three microorganisms: Escherichia coli, Klebsiella pneumoniae, and Zymomonas mobilis, were measured and compared and contrasted. A statistically comprehensive analysis of neutral red-mediated electrosynthesis is presented using the analysis of end-product profiles, current delivered, and changes in cellular protein expression. K. pneumoniae displayed the most dramatic response to electrosynthesis of the three bacteria, producing 93% more ethanol and 76% more lactate vs. control fermentation with no neutral red and no electron delivery. Z. mobilis showed no response to electrosynthesis except elevated acetate titers. Stoichiometric comparison showed that NAD+ reduction by neutral red could not account for changes in metabolites during electrosynthesis. Neutral red-mediated electrosynthesis was shown to have multifarious effects on the three species.
Journal: Bioresource Technology - Volume 195, November 2015, Pages 57–65