کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
679843 1459958 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of pretreatment methods on the synergism of cellulase and xylanase during the hydrolysis of bagasse
ترجمه فارسی عنوان
اثر روش های پیش درمان بر ترکیبات سلولاز و زایلاناز در طی هیدرولیز باگاس
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
چکیده انگلیسی


• Pretreatments resulted in different compositional and structural changes on bagasse.
• Presence of arabinan inhibited xylanase action on pretreated bagasse.
• Cellulose crystallinity and hemicellulose structure impacted on enzyme synergism.
• Variation of enzyme molecular structure affected the synergistic action.

The effect of pretreatment with peracetic acid (PAA) or an ionic liquid (1-ethyl-3-methylimidazolium acetate, [Emim][OAc]) on the synergism between endoglucanase and endoxylanase in the hydrolysis of bagasse was investigated. An endoglucanase, Cel6A, with a carbohydrate-binding module (CBM) and two endoxylanases, XynZ-C without a CBM and Xyn11A with an intrinsic xylan/cellulose binding module (XBM), were selected. The hemicellulose content, especially arabinan, and the cellulose crystallinity of bagasse were found to affect the cellulase–xylanase synergism. More specifically, higher synergism (above 3.4) was observed for glucan conversion, at low levels of arabinan (0.9%), during the hydrolysis of PAA pretreated bagasse. In contrast, [Emim][OAc] pretreated bagasse, showed lower cellulose crystallinity and achieved higher synergism (over 1.9) for xylan conversion. Ultimately, the combination of Cel6A and Xyn11A resulted in higher synergism for glucan conversion than the combination of Cel6A with XynZ-C, indicating the importance of the molecular architecture of enzymes for metabolic synergism.

Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 185, June 2015, Pages 158–164
نویسندگان
, , , , , , , ,