کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
680460 | 1459973 | 2014 | 6 صفحه PDF | دانلود رایگان |
• CO2 bubble size influenced carbon uptake in Arthrospira platensis KMMCC CY-007.
• mRNA expression levels varied according to uptake complexes and CO2 bubble size.
• Low affinity NDH-I4 complex was strongly expressed under fine bubble.
• NDH-I3 and also bicA and sbtA systems were weakly expressed under fine bubble.
Optimisation of cyanobacterial cell productivity should consider the key factors light cycle and carbon source. We studied the influence of CO2 bubble size on carbon uptake and fixation, on basis of mRNA expression levels in Arthrospira platensis KMMCC CY-007 at 30 °C (light intensity: 40 μmol m−2 s−1; 1% CO2). Growth rate, carbon fixation and lipid accumulation were examined over 7 days under fine bubble (FB) (100 μm Ø) bulk bubble (BB) (5000 μm Ø) and non-CO2 (NB) aeration. The low affinity CO2 uptake mRNA (NDH-I4 complex) was stronger expressed than the high affinity NDH-I3 complex (bicA and sbtA) under 1% CO2 and FB conditions, with no expression of bicA1 and sbtA1 after 4 days. The high affinity CO2 uptake mRNA levels corresponded to biomass, carbon content and lipid accumulation, and increase in NDH-I3 complex (9.72-fold), bicA (5.69-fold), and sbtA (10.61-fold), compared to NB, or BB conditions.
Journal: Bioresource Technology - Volume 170, October 2014, Pages 310–315