کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
680470 | 1459973 | 2014 | 8 صفحه PDF | دانلود رایگان |
• Carob pod extracts were used for succinic acid production by A. succinogenes.
• Kinetic studies of sucrose, glucose and fructose consumption were performed.
• Batch fermentations of extracts resulted in productivity of 1.61 g SA/L.h.
• Phenolic compounds present in extracts had no effect on succinic acid production.
Carob pods are a by-product of locust bean gum industry containing more than 50% (w/w) sucrose, glucose and fructose. In this work, carob pod water extracts were used, for the first time, for succinic acid production by Actinobacillus succinogenes 130Z. Kinetic studies of glucose, fructose and sucrose consumption as individual carbon sources till 30 g/L showed no inhibition on cell growth, sugar consumption and SA production rates. Sugar extraction from carob pods was optimized varying solid/liquid ratio and extraction time, maximizing sugar recovery while minimizing the extraction of polyphenols. Batch fermentations containing 10–15 g/L total sugars resulted in a maximum specific SA production rate of 0.61 Cmol/Cmol X.h, with a yield of 0.55 Cmol SA/Cmol sugar and a volumetric productivity of 1.61 g SA/L.h. Results demonstrate that carob pods can be a promising low cost feedstock for bio-based SA production.
Journal: Bioresource Technology - Volume 170, October 2014, Pages 491–498