کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
680734 | 1459990 | 2014 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Hydroxylation and hydrolysis: Two main metabolic ways of spiramycin I in anaerobic digestion Hydroxylation and hydrolysis: Two main metabolic ways of spiramycin I in anaerobic digestion](/preview/png/680734.png)
• Antibiotic like macrolide could be degraded in anaerobic digestion.
• Over 95% spiramycin was degraded in 32 day’s anaerobic digestion.
• Hydroxylation and hydrolysis were two metabolic ways of SPM I in anaerobic process.
• Structural analysis confirmed that P-3 was a new compound.
The anaerobic degradation behaviors of five macrolides including spiramycin I, II, III, midecamycin and josamycin by sludge were investigated. Within 32 days, 95% of spiramycin I, II or III was degraded, while the remove rate of midecamycin or josamycin was 75%. SPM I degradation was much higher in nutrition supplementation than that just in sludge. The degradation products and processes of spiramycin I were further characterized. Three molecules, designated P-1, P-2 and P-3 according to their order of occurrence, were obtained and purified. Structural determination was then performed by nuclear magnetic resonance and MS/MS spectra, and data indicated that hydroxylation and hydrolysis were main reactions during the anaerobic digestion of spiramycin I. P-1 is the intermediate of hydroxylation, and P-2 is the intermediate of hydrolysis. P-3 is the final product of the both reaction. This study revealed a hydroxylation and hydrolysis mechanism of macrolide in anaerobic digestion.
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Journal: Bioresource Technology - Volume 153, February 2014, Pages 95–100