کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
681187 | 1460016 | 2013 | 6 صفحه PDF | دانلود رایگان |

A bacterial strain, Cupriavidus sp. DT-1, capable of degrading chlorpyrifos and 3,5,6-trichloro-2-pyridinol (TCP) and using these compounds as sole carbon source was isolated and characterized. Investigation of the degradation pathway showed that chlorpyrifos was first hydrolyzed to TCP, successively dechlorinated to 2-pyridinol, and then subjected to the cleavage of the pyridine ring and further degradation. The mpd gene, encoding the enzyme responsible for chlorpyrifos hydrolysis to TCP, was cloned and expressed in Escherichia coli BL21. Inoculation of chlorpyrifos-contaminated soil with strain DT-1 resulted in a degradation rate of chlorpyrifos and TCP of 100% and 94.3%, respectively as compared to a rate of 28.2% and 19.9% in uninoculated soil. This finding suggests that strain DT-1 has potential for use in bioremediation of chlorpyrifos-contaminated environments.
► A chlorpyrifos- and 3,5,6-trichloro-2-pyridinol (TCP)-degrading strain (DT-1) belonging to the genus Cupriavidus was isolated.
► The biodegradation pathway of TCP was examined.
► The mpd gene encoding an organophosphorus hydrolase was cloned.
► Strain DT-1 promotes the degradation of chlorpyrifos and TCP in soil.
► Strain DT-1 is a good candidate for studying the degradation mechanism of TCP.
Journal: Bioresource Technology - Volume 127, January 2013, Pages 337–342