کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
681555 | 1460028 | 2012 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Biocatalytic production of novel glycolipids with cellodextrin phosphorylase Biocatalytic production of novel glycolipids with cellodextrin phosphorylase](/preview/png/681555.png)
Glycolipids have gained increasing attention as natural surfactants with a beneficial environmental profile. They are typically produced by fermentation, which only gives access to a limited number of structures. Here we describe the biocatalytic production of novel glycolipids with the cellodextrin phosphorylase from Clostridium stercorarium. This enzyme was found to display a broad donor and acceptor specificity, allowing the synthesis of five different products. Indeed, using either α-glucose 1-phosphate or α-galactose 1-phosphate as glycosyl donor, sophorolipid as well as glucolipid could be efficiently glycosylated. The transfer of a glucosyl moiety afforded a mixture of products that precipitated from the solution, resulting in near quantitative yields. The transfer of a galactosyl moiety, in contrast, generated a single product that remained in solution at thermodynamic equilibrium. These glycolipids not only serve as a new class of biosurfactants, but could also have applications in the pharmaceutical and nanomaterials industries.
► Cellodextrin phosphorylase from Clostridium stercorarium is able to synthesize new glycolipids.
► Glucolipid and sophorolipid acceptors are extended with one or two glucosyl moieties.
► Product precipitation drives the reactions to near completion and facilitates purification.
► The glycosyl donor α-galactose 1-phosphate allows the synthesis of lactolipid in highly pure form.
Journal: Bioresource Technology - Volume 115, July 2012, Pages 84–87